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Gaseous Oxides and Olfactory Computation Flipbook PDF
GASEOUS OXIDES AND OLFACTION 333 plasticity in a variety of systems (Nighorn et al., 1998; Cramer and Sur, 1999; Renteri
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AMER. ZOOL., 41:332–345 (2001)
Gaseous Oxides and Olfactory Computation1 A. GELPERIN,2 J. P. Y. KAO,3
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I. R. C. COOKE4
Biological Computation Research Department Bell Laboratories, Lucent Technologies, 600 Mountain Avenue, Murray Hill, New Jersey 07974
INTRODUCTION Olfactory systems are remarkable both for the sensitivity and scope of their molecular detection ability and for the plasticity of their synaptic programming. At the limits of odor sensitivity only a few dozen molecules are sufficient for detection and categorization of the odor sampled by the receptor surface. Synaptic plasticity in olfaction is evident both in the way odor cues participate in higher-order associative learning (Sahley, 1990; Eichenbaum, 1998) and in the maintenance of olfactory function 1 From the Symposium on Nitric Oxide in the Invertebrates: Comparative Physiology and Diverse Functions presented at the Annual Meeting of the Society for Integrative and Comparative Biology, 4–8 January 2000, at Atlanta, Georgia. 2 E-mail: [email protected] 3 Present address of J. P. Y. Kao is Medical Biotechnology Center, University of Maryland School of Medicine, Baltimore, Maryland 21201. 4 Present address of I. R. C. Cooke is Macfarlane Burnet Centre for Medical Research, Fairfield, Victoria, Australia 3078.
during continual postembryonic neurogenesis at the sensory periphery (Chase and Rieling, 1986; Sandeman and Sandeman, 1996; Weiler and Farbman, 1997; Weiler et al., 1999) and in central odor processing sites (Harzsch et al., 1999; Sandeman et al., 1998; Zakharov et al., 1998; Kirschenbaum et al., 1999; Murray and Calof, 1999; Mellon and Tewari, 2000). Gaseous neurotransmitters such as nitric oxide (NO) and carbon monoxide (CO) are prominent and universal constituents of the array of neurotransmitters found in olfactory systems, both centrally and at the receptor surface (Breer and Shepherd, 1993; Muller and Hildebrandt, 1995; Hildebrand and Shepherd, 1997; Zufall and LeindersZufall, 1997; Gelperin, 1999; Koh and Jacklet, 1999; Moroz, 2001). There is evidence that NO and CO play a role in olfactory synaptic plasticity (Robertson et al., 1994, 1995; Mu¨ller, 1996; Okere et al., 1996; Teyke, 1996; Kendrick et al., 1997) as well as developmental guidance and
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SYNOPSIS. The gaseous neurotransmitters nitric oxide (NO) and carbon monoxide (CO) are prominent and universal components of the array of neurotransmitters found in olfactory information processing systems. These highly mobile communication compounds have effects on both second messenger signaling and directly on ion channel gating in olfactory receptors and central synaptic processing of receptor input. Olfactory systems are notable for the plasticity of their synaptic connections, revealed both in higher-order associative learning mechanisms using odor cues and developmental plasticity operating to maintain function during addition of new olfactory receptors and new central olfactory interneurons. We use the macrosmatic terrestrial mollusk Limax maximus to investigate the role of NO and CO in the dynamics of central odor processing and odor learning. The major central site of odor processing in the Limax CNS is the procerebral (PC) lobe of the cerebral ganglion, which displays oscillatory dynamics of its local field potential and periodic activity waves modulated by odor input. The bursting neurons in the PC lobe are dependent on local NO synthesis for maintenance of bursting activity and wave propagation. New data show that these bursting PC interneurons are also stimulated by carbon monoxide. The synthesizing enzyme for carbon monoxide, heme oxygenase 2, is present in the neuropil of the PC lobe. Since the PC lobe exhibits two forms of synaptic plasticity related to both associative odor learning and continual connection of new receptors and interneurons, the use of multiple gaseous neurotransmitters may be required to enable these multiple forms of synaptic plasticity.
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recorded with saline-filled glass electrodes in vitro is also characteristic of some periods of LFP recordings made with implanted fine-wire electrodes in vivo (Cooke and Gelperin, 2001). RESULTS NO and CO production in the PC lobe Because the anatomy of the olfactory information processing pathways in Limax and Helix strongly suggests that the PC lobe is the major central site of olfactory processing, we wished to assess the presence of synthesizing enzymes for NO and CO in the PC lobe. The NADPH diaphorase reaction for nitric oxide synthase (NOS) gives a very prominent reaction product in the neuropil regions of the PC lobe and in some cells and fiber tracts within the cell layer (Fig. 2A) (Cooke et al., 1994; Sa´nchez-Alvarez et al., 1994; Gelperin et al., 2000). The PC lobe has two distinct neuropil regions, the internal mass (IM) at the base of the lobe where neurites of intrinsic PC interneurons interact, and the terminal mass (TM) adjacent to the cell layer, where processes of the olfactory afferent fibers interact with neurites of intrinsic PC interneurons (Kawahara et al., 1997; Ratte´ and Chase, 1997). The tentacular ganglion immediately adjacent to the olfactory receptor surface also reacts strongly for NADPH diaphorase activity (Fig. 2B). Approximately 15% of the olfactory receptors synapse in the tentacular ganglion (Chase and Tolloczko, 1993) hence some of the input to the PC lobe may be second order. CO is synthesized in neural tissue by heme oxygenase 2 (HO2), which reacts with heme to generate CO, iron, and biliverdin, which is immediately reduced to bilirubin (Maines, 1993; Dore et al., 1999). Using a polyclonal antibody to rat native HO2 (StressGen Biotechnologies, Victoria, CA), we consistently found (six preparations) a discrete region of punctate staining at the base of the IM neuropil in the Limax PC lobe (Fig. 3A) and in the PC lobe of Helix (Fig. 3B). The cells of origin for the fiber staining shown in Figure 3 are at present unknown. There are, however, numerous HO2 immunoreactive somata outside
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plasticity in a variety of systems (Nighorn et al., 1998; Cramer and Sur, 1999; Renteria and Constantine-Paton, 1999; Schachtner et al., 1999; Van Wagenen and Rehder, 1999; Wildemann and Bicker, 1999). More generally, gaseous neurotransmitters, particularly NO, are prime candidates for a feedback signal from postsynaptic to presynaptic compartments mediating synaptic plasticity and Hebbian learning in mammals (Altememi and Alkadhi, 1999; Haul et al., 1999; Ko and Kelly, 1999; Lu et al., 1999; Moody et al., 1999; Murphy and Bliss, 1999; Wilson et al., 1999; Zhuo et al., 1999) and mollusks (Bravarenko et al., 1995; Teyke, 1996; Fossier et al., 1999; Malyshev and Balaban, 1999) We are investigating the role of small mobile neurotransmitters such as NO and CO in the olfactory system of the terrestrial mollusk Limax maximus, a macrosmatic species with highly developed odor learning ability (Sahley, 1990; Sekiguchi et al., 1997; Kimura et al., 1998b; Gelperin, 1999), also well described in Helix (Balaban et al., 1994; Teyke, 1995) The olfactory systems of Limax and Helix display a number of general design features found in mammalian olfaction, including continual receptor turnover (Chase and Rieling, 1986) and postembryonic neurogenesis in central olfactory processing sites (Zakharov et al., 1998). The major central site of odor processing is the procerebral (PC) lobe of the cerebral ganglion, which receives direct input from receptors (Zaitseva, 1991; Chase and Tolloczko, 1993). The PC lobe has oscillatory dynamics evident in the periodicity of its local field potential (Gelperin and Tank, 1990; Kleinfeld et al., 1994; Kawahara et al., 1997; Kimura et al., 1998a) and supports activity wave propagation from its apex to base at approximately 1 Hz (Ermentrout et al., 1998; Inoue et al., 1998; Kimura et al., 1998a; Nikitin and Balaban, 1999). The oscillatory nature of LFP recordings in the PC lobe arises from the periodicity of wave propagation past the recording site. If LFP recordings are made simultaneously from apex and base, a phase delay is measured during wave propagation in both Limax (Ermentrout et al., 1998) and Helix (Fig. 1). The oscillatory LFP activity
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the PC lobe, particularly in the subesophageal ganglia (Figs. 4A, B, 5). Since we know that pedal and buccal cells have neurites in the PC lobe (Chase and Tolloczko, 1989; Gelperin and Flores, 1997; Ratte´ and Chase, 1997), it is quite plausible that the somata belonging to the HO2 immunoreactive fibers at the base of the IM neuropil are in the subesophageal ganglia. As with NOS staining, the tentacular (5digitate) ganglion also shows clear staining for HO2 in both Helix (Fig. 6) and Limax (data not shown). We have not examined the buccal ganglia for HO2 staining. The wide distribution of HO2 immunoreactive cells and fibers in the Helix CNS strongly suggests that
CO may play a substantial role in neuronal communication in a variety of CNS circuits in Helix and in other terrestrial gastropods. NO production is necessary for LFP oscillation in the PC lobe We have shown previously that inhibition of NOS slows and can stop the cellular activity responsible for the LFP oscillation in the PC lobe (Gelperin, 1994a; Gelperin et al., 2000). Application of either oxyheme proteins as extracellular NO scavengers or inhibitors of NOS such as substituted arginines can reversibly halt the LFP oscillation. Intracellular recordings from the bursting PC neurons responsible for the LFP os-
← FIG. 1. The procerebral lobe of Helix aspersa shows spontaneous ongoing oscillations of local field potential (LFP) and phase delays in the peak of the LFP from apical to basal regions. The LFP is recorded using a salinefilled glass electrode inserted into the cell layer of the PC lobe and connected to a current-to-voltage converter. A. Dual recordings of the local field potential in the procerebral lobe of Helix show periodic currents at both sites which are phase locked at a frequency of 0.52 Hz. B. The phase delay for the peak of the local field potential event between apical and basal recording sites separated by 40 mm is 50 msec, corresponding to a propagation speed of 0.8 mm/msec.
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FIG. 2. NADPH diaphorase activity in the olfactory regions of the nervous system of Limax. A. NADPH diaphorase activity in the procerebral lobe. Note the very intense staining of the internal mass neuropil (IM), the strong staining of the terminal mass neuropil (TM) and the staining of fiber tracts within the cell body layers (C). B. NADPH diaphorase activity in the tentacular ganglion of the superior tentacle. Strong NADPH diaphorase activity occurred in the major neuropil region of the ganglion (N), less intense staining occurred in regions of neuropil within the cell body layers and in the projections of receptor cells beneath the sensory epithelium (SE) of the nose
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cillation and wave propagation (Kleinfeld et al., 1994) show that they are stimulated by NO application and become quiescent upon inhibition of NO synthesis (Gelperin et al., 2000). The caged NO compound nitrosylpentachlororuthenate (NPR) (Murphy and Bliss, 1999) provides a particularly convenient and effective means of NO application. Using PC lobe preparations bathed in NPR and very localized uncaging flashes, we found that small regions (30–50 mm diameter) of the PC lobe can be stimulated to produce high frequency activity independent of the ongoing macroscopic LFP oscillation and wave propagation (Fig. 7). Flashes delivered in the absence of NPR are without effect. This is consistent with the possibility that localized afferent input aris-
FIG. 4A. HO2 immunoreactivity in the procerebral lobe of Helix. A discrete region of punctate staining (arrow) was always observed at the base of the internal mass neuropil (IM). A similarly discrete region of strong HO2 immunoreactivity was always observed in the same region of the PC lobe in Limax. TM: terminal mass neuropil, C: cell body layer.
ing from odor stimulation could stimulate local activity in the PC lobe not evident in the macroscopic measurement of the oscillating LFP (Teyke and Gelperin, 1999). CO application alters LFP oscillation frequency A new reagent for temporally and spatially controlled application of CO to neural tissue has recently been synthesized (Kao and Keitz, 1997). This molecule, NV/CO, is shown in Figure 8A, while the photolytic reaction triggered by near UV stimulation is shown in Figure 8B. Calibration of NV/ CO using rat aortic smooth muscle cells and measuring cGMP content shows the activity of NV/CO to parallel that obtained with CO-containing solutions (Kao and Keitz,
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FIG. 3. HO2 immunoreactivity in the procerebral lobe of Limax A discrete region of punctate staining (arrow) was always observed at the base of the internal mass neuropil (IM). A similarly discrete region of strong HO2 immunoreactivity was always observed in the same region of the PC lobe in Helix. TM: terminal mass neuropil, C: cell body layer.
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FIG. 4B. HO2 immunoreactivity in ganglia and connectives in Helix. Low power view of an oblique section through all of the subesophageal ganglia showing the presence of HO2 immunoreactivity in isolated cells and clusters of cells in each ganglion. V: visceral ganglion; Pa: parietal ganglion; Pl: pleural ganglion; Pe: pedal ganglion.
1997). We have found that CO application to Limax PC lobes using flash photolysis of NV/CO increases the frequency of the ongoing LFP oscillation (Fig. 9) in a dosedependent manner (Fig. 10). Since the caged CO compound also liberates benzene upon photolysis, control experiments were
done applying benzene at 10, 20 and 50 mM to the PC lobe with either no effect or a slight reduction in LFP oscillation frequency (data not shown). These concentrations span a range more than 10 fold higher that the calculated peak concentration of benzene resulting from uncaging of NV-CO.
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DISCUSSION The PC lobe of Limax appears to use two gaseous neurotransmitters, NO and CO, in maintaining its normal oscillatory dynamics and wave propagating mode (Gelperin, 1994b, 1999; Gelperin et al., 1996). Two lines of evidence suggest that maintenance of oscillatory dynamics and wave propa-
gation in the Limax PC lobe is necessary for odor learning and odor discrimination. On the assumption that structure and dynamics of the Helix PC lobe are very similar to those of Limax (cf., Fig. 1 and [Ratte´ and Chase, 2000]), the finding that injecting an inhibitor of NOS into Helix blocks odor learning (Teyke, 1996) could be interpreted
FIG. 6. HO2 immunoreactivity in the digitate ganglion of the superior tentacle and in the tentacular nerve of Helix. Left panel: Low power view of a vertical section through the nose of a retracted tentacle showing the sensory epithelium of the nose (SE) and the cell body layers (C) and neuropil (N) of the digitate ganglion. A discrete region of HO2 immunoreactive neuropil in the digitate ganglion is outlined by the white box and shown at higher power in the middle panel. This strongly staining discrete region of neuropil was a reliable feature of Helix preparations. A similarly located region of HO2-immunoreactive neuropil was seen in Limax, but staining was not as strong. Right panel: HO2-immunoreactive fibers in the tentacular nerve of Helix.
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FIG. 5. HO2-immunoreactive cells in the subesophageal ganglia of Helix. These are photomicrographs at higher magnification of groups of cells evident in the low magnification view of the section through all of the subesophageal ganglia in Figure 4. Left panel: Cluster of HO2-immunoreactive cells in the visceral ganglion. Note also the punctate staining of the neuropil. Right panel: Several HO2-immunoreactive cells in a pedal ganglion.
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to mean that blocking the oscillation and wave propagation of the Helix PC lobe blocks odor learning. The Helix PC lobe stains densely for NOS (Cooke et al., 1994) and, as in Limax, has odor-modulated wave propagation from apex to base (Nikitin and Balaban, 2000). A critical test of this interpretation is to measure the effect of the injected NOS inhibitor on PC lobe activity using implanted electrodes in the intact animal, which has recently been demonstrated (Cooke and Gelperin, 2001). Another type of evidence linking PC oscillations and wave propagation to odor memory access was obtained from odor-elicited responses in the peritentacular nerve (PTN) of Limax studied in nose-brain preparations prepared from pretrained slugs (Teyke and Gelperin, 1999). The PTN discharge is specific to odors which have been appetitively conditioned (Peschel et al., 1996). Reversibly blocking the oscillations in the PC lobe did not impair recognition of a conditioned odor but did reduce the differential PTN response to closely related odors (Teyke and Gelperin, 1999). This suggests that blocking the PC lobe oscillation in vivo would
degrade odor discrimination between similar odors, which has been observed in honeybee after blocking odor-elicited oscillations in the antennal lobe and mushroom body by the in vivo application of picrotoxin (Stopfer et al., 1997). Odor-elicited oscillations are a general feature of olfactory systems (Adrian, 1942; Hughes and Mazurowski, 1962; Hughes et al., 1969; Laurent and Naraghi, 1994; Delaney and Hall, 1996; Dorries and Kauer, 2000) and there is great interest in deciphering their computational role (Gray, 1994; Gelperin, 1999; Hopfield, 1999; Lam et al., 2000). The odor-elicited oscillations in mammalian olfactory bulb may be mediated or augmented by NO, as the olfactory bulb stains densely for NOS (Bredt et al., 1991; Vincent and Kimura, 1992; ElHusseini et al., 1999; Nakamura et al., 1999) and NO affects the excitability of mammalian central neurons (Pape and Mager, 1992; Pineda et al., 1996; Yang and Hatton, 1999). NO acts within a volume centered on its site of synthesis with radius of action dependent on the geometry of its source as well as the types and abundance
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FIG. 7. Localized photolysis of caged nitric oxide can elicit localized high frequency oscillations which do not propagate along the apical-basal axis of the Limax procerebral lobe and do not reset the macroscopic oscillation frequency. A 200-mm thick slice of the procerebral lobe was bathed in 50 mm caged NO (NPR) for 45 min and then rinsed with saline. The uncaging flash was 70 msec in duration and 35 mm in diameter centered on the apical recording electrode.
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FIG. 8B.
of molecules which bind or inactivate NO (Philippides et al., 2000). Nitric oxide is known to affect growth and migration of neurons and glial cells (Cramer and Sur, 1999; Renteria and Constantine-Paton, 1999; Van Wagenen and Rehder, 1999; Chen et al., 2000). Since the postembryonic PC lobe continually receives new input fibers from newly generated receptors (Chase and Rieling, 1986) and generates new neurons for at least several months after hatching (Zakharov et al., 1998), NO may play a role in the plasticity required to integrate these new synapses into the existing PC lobe circuitry. Carbon monoxide, like NO, is a small highly mobile neurotransmitter with a clear association with the olfactory system. Olfactory receptor neurons and elements of the olfactory bulb in mammals have the highest concentration of HO2 in the entire mammalian CNS (Ewing et al., 1993; Verma et al., 1993). Studies on olfactory receptors provide particularly clear evidence for a role for CO in responses to odor ligands (Ingi and Ronnett, 1995; Ingi et al., 1996a, b; Zufall and Leinders-Zufall, 1997). The role of CO in central olfactory circuits is as yet unknown. CO in the Limax PC lobe may be involved in setting the burster neuron oscillation frequency and hence the frequency of the LFP oscillation or in mechanisms of synaptic plasticity for odor learning or connectional plasticity due
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FIG. 8A. The molecular structure and photochemical reaction of NV/CO, a caged carbon monoxide compound. A. The molecular structure of NV/CO. The nature of the substituent at the position denoted by R determines if the compound is membrane permeant (R 5 AM ester) or not (R 5 K or Na). B. Photolytic reaction of NV/CO. Step 1: absorption of UV light causes an oxidation-reduction reaction within the caged CO molecule: an oxygen atom is transferred from the nitro group (2NO2) to the carbon that is adjacent to the benzene ring bearing the nitro. Step 2: The product, b, of this photochemical reaction rearranges to yield two molecular fragments—c and d. Step 3: Fragment d is extremely unstable and rapidly breaks down into a molecule of benzene (e) and a molecule of carbon monoxide (f).
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to new synapse formation during adult neurogenesis. Experiments to probe the role of endogenous CO production in the PC lobe are complicated by the need to selectively
inhibit HO2 without inhibiting NOS or soluble guanylyl cyclase (Meffert et al., 1994). In some systems selective inhibition of HO2 can be accomplished by applying ap-
FIG. 10. Dose-response curve relating peak increase in local field potential oscillation frequency to the duration of the NV/CO uncaging flash. Each uncaging flash duration was repeated 10 times. The 3 cycles of the LFP response with highest frequency were taken for each trial and the 30 values averaged and plotted 6SD. Values at flash durations from 10 to 300 msec are significantly different from their control prestimulation values (Student’s t-test, two tailed, P , 0.05).
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FIG. 9. Application of carbon monoxide to a slice preparation of the procerebral lobe of Limax increases the frequency of the local field potential oscillation. The slice was bathed in 44 mM NV/CO-AM. Photolysis was stimulated with an uncaging flash of 360 6 10 nm light covering 75% of the preparation.
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propriate concentrations of chromium mesoporphyrin IX (Appleton et al., 1999). In cultures of cerebellar granule cells, CO modulates the NO-cGMP signaling system (Ingi et al., 1996a). The interactions between NO and CO generating systems have been reviewed recently (Maines, 1997). ACKNOWLEDGMENTS We thank J. W. Wang and J. Flores for collecting some of the data used in this paper. REFERENCES
thase in the nervous system of the pulmonate mollusc Helix aspersa. Cell Tissue Research 277: 565–572. Cooke, I. R. C. and A. Gelperin. 2001. In vivo recordings of spontaneous and odor-modulated dynamics in the Limax olfactory lobe. J. Neurobiol. 46: 126–141. Cramer, K. S. and M. Sur. 1999. The neuronal form of nitric oxide synthase is required for pattern formation by retinal afferents in the ferret lateral geniculate nucleus. Develop. Brain Research 116: 79–86. Delaney, K. R. and J. B. Hall. 1996. An in vitro preparation of frog nose and brain for the study of odour-evoked oscillatory activity. J. Neurosci. Methods 68:193–202. Dore, S., M. Takahashi, C. D. Ferris, L. D. Hester, D. Guastella, and S. H. Snyder. 1999. Bilirubin, formed by activation of heme oxygenase-2, protects neurons against oxidative stress. Proc. Natl. Acad. Sci. U.S.A. 96:2445–2450. Dorries, K. M. and J. S. Kauer. 2000. Relationship between odor-elicited olscillations in the salamander olfactory epithelium and olfactory bulb. J. Neurophysiol 83:754–765. Eichenbaum, H. 1998. Using olfaction to study memory. Ann. N.Y. Acad. Sci. 855:657–669. El-Husseini, A. E. D., J. Williams, P. B. Reiner, S. Pelech, and S. R. Vincent. 1999. Localization of the cGMP-dependent protein kinases in relation to nitric oxide synthase in the brain. J. Chem. Neuroanatomy 17:45–55. Ermentrout, B., J. Flores, and A. Gelperin. 1998. Minimal model of oscillations and waves in the Limax olfactory lobe with tests of the model’s predictive power. J. Neurophysiol. 79:2677–2689. Ewing, J., C. Weber, and M. Maines. 1993. Biliverdin reductase is heat resistant and coexpressed with constitutive and heat shock forms of heme oxygenase in brain. J. Neurochem. 61:1015–1023. Fossier, P., B. Blanchard, C. Ducrocq, C. Leprince, L. Tauc, and G. Baux. 1999. Nitric oxide transforms serotonin into an inactive form and this affects neuromodulation. Neuroscience 93:597–603. Gelperin, A. 1994a. Nitric oxide mediates network oscillations of olfactory interneurons in a terrestrial mollusc. Nature 369:61–63. Gelperin, A. 1994b. Nitric oxide, odor processing and plasticity. Netherl. J. Zool. 44:159–169. Gelperin, A. 1999. Oscillatory dynamics and information processing in olfactory systems. J. Exp. Biol 202:1855–1864. Gelperin, A. and J. Flores. 1997. Vital staining from dye-coated microprobes identifies new olfactory interneurons for optical and electrical recording. J. Neurosci. Methods 72:97–108. Gelperin, A., J. Flores, F. Raccuia-Behling, and I. R. C. Cooke. 2000. Nitric oxide and carbon monoxide modulate oscillations of olfactory interneurons in a terrestrial mollusc. J. Neurophysiol. 83:116– 127. Gelperin, A., D. Kleinfeld, W. Denk, and I. R. C. Cooke. 1996. Oscillations and gaseous oxides in invertebrate olfaction. J. Neurobiol. 30:110–122.
Downloaded from http://icb.oxfordjournals.org/ by guest on June 22, 2015
Adrian, E. D. 1942. Olfactory reactions in the brain of the hedgehog. J. Physiol. London 100:459–473. Altememi, G. F. and K. A. Alkadhi. 1999. Nitric oxide is required for the maintenance but not initiation of ganglionic long-term potentiation. Neuroscience 94:897–902. Appleton, S. D., M. L. Chretien, B. E. McLaughlin, H. J. Vreman, D. K. Stevenson, J. F. Brien, K. Nakatsu, D. H. Maurice, and G. S. Marks. 1999. Selective inhibition of heme oxygenase, without inhibition of nitric oxide synthase or soluble guanylyl cyclase, by metalloporphyrins at low concentrations. Drug Metab. Dispos. 27:1214–1219. Balaban, P. M., O. A. Masimova, and H. I. Bravarenko. 1994. Behavioral plasticity in a snail and its neural mechanisms. Neurosci. Behav. Physiol. 24:97– 104. Bravarenko, N. I., P. V. Gusev, P. M. Balaban, and L. L. Voronin. 1995. Postsynaptic induction of longterm synaptic facilitation in snail central neurons. Neuroreport 6:1182–1186. Bredt, D. S., C. E. Glatt, P. M. Hwang, M. Fotuhi, T. M. Dawson, and S. H. Snyder. 1991. Nitric oxide synthase protein and mRNA are discretely localized in neuronal populations of the mammalian CNS together with NADPH diaphorase. Neuron 7:615–624. Breer, H. and G. M. Shepherd. 1993. Implications of the NO/cGMP system for olfaction. TINS 16:5–9. Chase, R. and J. Rieling. 1986. Autoradiographic evidence for receptor cell renewal in the olfactory epithelium of a snail. Brain Res. 384:232–239. Chase, R. and B. Tolloczko. 1989. Interganglionic dendrites constitute an output pathway from the procerebrum of the snail Achatina fulica. J. Comp. Neurol. 283:143–152. Chase, R. and B. Tolloczko. 1993. Tracing neural pathways in snail olfaction: From the tip of the tentacles to the brain and beyond. Micr. Res. Tech. 24:214–230. Chen, A., S. M. Kumar, C. L. Sahley, and K. J. Muller. 2000. Nitric oxide influences injury-induced microglial migration and accumulation in the leech CNS. J. Neurosci. 20:1036–1043. Cooke, I. R. C., S. L. Edwards, and C. R. Anderson. 1994. The distribution of NADPH-diaphorase activity and immunoreactivity to nitric oxide syn-
ET AL.
GASEOUS OXIDES
OLFACTION
343
factory response of the procerebral lobe in the slug brain. Learning & Memory 4:289–400. Kimura, T., S. Toda, T. Sekiguchi, and Y. Kirino. 1998b. Behavioral modulation induced by food odor aversive conditioning and its influence on the olfactory responses of an oscillatory brain network in the slug Limax marginatus. Learning & Memory 4:365–375. Kirschenbaum, B., F. Doetsch, C. Lois, and A. Alvarez-Buylla. 1999. Adult subventricular zone neuronal precursors continue to proliferate and migrate in the absence of the olfactory bulb. J. Neurosci. 19:2171–2180. Kleinfeld, D., K. R. Delaney, M. S. Fee, J. A. Flores, D. W. Tank, and A. Gelperin. 1994. Dynamics of propagating waves in the olfactory network of a terrestrial mollusc: An electrical and optical study. J. Neurophysiol. 72:1402–1419. Ko, G. Y. and P. T. Kelly. 1999. Nitric oxide acts as a postsynaptic signaling molecule in calcium/calmodulin-induced synaptic potentiation in hippocampal CA1 neurons. J. Neurosci. 19:6784–6794. Koh, H. Y. and J. W. Jacklet. 1999. Nitric oxide stimulates cGMP prodution and mimics synaptic responses in metacerebral neurons of Aplysia. J. Neurosci. 19:3818–3826. Lam, Y.-W., L. B. Cohen, M. Washowiak, and M. R. Zochowski. 2000. Odors elicit three different oscillations in the turtle olfactory bulb. J. Neurosci. 20:749–762. Laurent, G. and M. Naraghi. 1994. Odorant-induced oscillations in the mushroom bodies of the locust. J. Neurosci. 14:2993–3004. Lu, Y.-F., E. R. Kandel, and R. D. Hawkins. 1999. Nitric oxide signaling contributes to late-phase LTP and CREB phosphorylation in the hippocampus. J. Neurosci. 19:10250–10261. Maines, M. D. 1993. Carbon monoxide: An emerging regulator of cGMP in the brain. Molec. Cell. Neurosci. 4:389–397. Maines, M. D. 1997. The heme oxygenase system: A regulator of second messenger gases. Annu. Rev. Pharmacol. Toxicol. 37:517–554. Malyshev, A. Y. and P. M. Balaban. 1999. Synaptic facilitation in Helix neurons depends upon postsynaptic calcium and nitric oxide. Neurosci. Lett. 216:65–68. Meffert, M. K., J. E. Haley, E. M. Schuman, H. Schulman, and D. V. Madison. 1994. Inhibition of hippocampal heme oxygenase, nitric oxide synthase, and long-term potentiation by metalloporphyrins. Neuron 13:1225–1233. Mellon, D. and M. K. Tewari. 2000. Heteromorphic antennules protect the olfactory midbrain from atrophy following chronic antennular ablation in frshwater crayfish. J. Exp. Zool. 286:90–96. Moody, T. D., H. J. Carlisle, and T. J. O’Dell. 1999. A nitric oxide-independent and beta-adrenergic receptor-sensitive form of metaplasticity limits theta-frequency stimulation-induced LTP in the hippocampal CA1 region. Learning & Memory 6: 619–633. Moroz, L. L. 2001. Gaseous transmission across time and species. Amer. Zool. 41:304–320.
Downloaded from http://icb.oxfordjournals.org/ by guest on June 22, 2015
Gelperin, A. and D. W. Tank. 1990. Odor-modulated collective network oscillations of olfactory interneurons in a terrestrial mollusc. Nature 345:437– 440. Gray, C. M. 1994. Synchronous oscillations in neuronal systems: Mechanisms and functions. J. Comput. Neurosci. 1:11–38. Harzsch, S., J. Miller, J. Benton, and B. Beltz. 1999. From embryo to adult: Persistent neurogenesis and apoptotic cell death shape the lobster deutocerebrum. J. Neurosci. 19:3472–3485. Haul, S., A. Godecke, J. Schrader, H. L. Haas, and H. J. Luhmann. 1999. Impairment of neocortical long-term potentiation in mice deficient of endothelial nitric oxide synthase. J. Neurophysiol. 81: 494–497. Hildebrand, J. G. and G. M. Shepherd. 1997. Mechanisms of olfactory discrimination: Converging evidence for common principles across phyla. Annu. Rev. Neurosci. 20:595–631. Hopfield, J. J. 1999. Odor space and olfactory processing: Collective algorithms and neural implementation. Proc. Natl. Acad. Sci. U.S.A. 96: 12506–12511. Hughes, J. R., D. E. Hendrix, and N. Wetzel. 1969. Electrophysiological studies of the olfactory bulb in man: A model for EEG investigation. Electroencephalogr. Clin. Neurophysiol. 27:661. Hughes, J. R. and J. A. Mazurowski. 1962. Studies on the supracallosal mesial cortex of unanesthetized, conscious mammals. II Monkey. B. Responses from the olfactory bulb. Electroencephalogr. Clin. Neurophysiol. 14:635–645. Ingi, T., J. Cheng, and G. V. Ronnett. 1996a. Carbon monoxide: An endogenous modulator of the nitric oxide cyclic GMP signaling system. Neuron 16: 835–842. Ingi, T., G. Chiang, and G. V. Ronnett. 1996b. The regulation of heme turnover and carbon monoxide biosynthesis in cultured primary rat olfactory receptor neurons. J. Neurosci. 16:5621–5628. Ingi, T. and G. V. Ronnett. 1995. Direct demonstration of a physiological role for carbon monoxide in olfactory receptor neurons. J. Neurosci. 15:8214– 8222. Inoue, T., S. Kawahara, S. Toda, S. Watanabe, and Y. Kirino. 1998. Selective optical recording of the neural activity in the olfactory center of land slug using a calcium indicator dye. Bioimages 6:59– 67. Kao, J. P. Y. and P. F. Keitz. 1997. Photosensitive organic compounds that release carbon monoxide upon illumination. U.S. Patent 5,670,664. Kawahara, S., S. Toda, Y. Suzuki, S. Watanabe, and Y. Kirino. 1997. Comparative study on neural oscillation in the procerebrum of the terrestrial slugs Incilaria bilineata and Limax marginatus. J. Exp. Biol. 200:1851–1861. Kendrick, K. M., R. Guevara-Guzman, J. Zorrilla, M. R. Hinton, K. D. Broad, M. Mimmack, and S. Ohkura. 1997. Formation of olfactory memories mediated by nitric oxide. Nature 388:670–674. Kimura, T., S. Toda, T. Sekiguchi, S. Kawahara, and Y. Kirino. 1998a. Optical recording analysis of ol-
AND
344
A. GELPERIN
a retrograde messenger during map refinement and segregation. J. Neurosci. 19:7066–7076. Robertson, J. D., J. Bonaventura, and A. Kohm. 1995. Nitric oxide synthase inhibition blocks octopus touch learning without producing sensory or motor dysfunction. Proc. R. Soc. London B 261:167– 172. Robertson, J. D., J. Bonaventura, and A. P. Kohm. 1994. Nitric oxide is required for tactile learning in Octopus vulgaris. Proc. R. Soc. London B 256: 269–273. Sahley, C. L. 1990. The behavioral analysis of associative learning in the terrestrial mollusc Limax maximus: The importance of interevent relationships. In S. Hanson and C. Olson (eds.), Connectionist modeling and brain function: The developing interface, pp. 36–73. MIT Press, Cambridge, MA. Sa´nchez-Alvarez, M., M. Leo´n-Olea, E. Talavera, F. Pellicer, E. Sa´nchez-Islas, and G. Martı ´nez-Lorenzana. 1994. Distribution of NADPH-diaphorase in the perioesophageal ganglia of the snail, Helix aspersa. Neurosci. Lett. 169:51–55. Sandeman, R., D. Clarke, D. Sandeman, and M. Manly. 1998. Growth-related and antennular amputation-induced changes in the olfactory centers of crayfish brain. J. Neurosci. 18:6195–6206. Sandeman, R. E. and D. C. Sandeman. 1996. Preembryonic and postembryonic development, growth and turnover of olfactory receptor neurons in crayfish antennules. J. Exp. Biol. 199:2409–2418. Schachtner, J., U. Homberg, and J. W. Truman. 1999. Regulation of cyclic GMP elevation in the developing antennal lobe of the Sphinx moth, Manduca sexta. J. Neurobiol. 41:359–375. Sekiguchi, T., A. Yamada, and H. Suzuki. 1997. Reactivation dependent changes in memory states in the terrestrial slug Limax flavus. Learning & Memory 4:356–364. Stopfer, M., S. Bhagavan, B. H. Smith, and G. Laurent. 1997. Impaired odour discrimination on desynchronization of odour-encoding neural assemblies. Nature 390:70–74. Teyke, T. 1995. Food-attraction conditioning in the Roman snail, Helix pomatia. J. Comp. Physiol. A 177:409–414. Teyke, T. 1996. Nitric oxide, but not serotonin, is involved in acquisition of food-attraction conditioning in the snail Helix pomatia. Neurosci. Lett. 206:29–32. Teyke, T. and A. Gelperin. 1999. Olfactory oscillations augment odor discrimination not odor identification by Limax CNS. NeuroReport 10:1061–1068. Van Wagenen, S. and V. Rehder. 1999. Regulation of neuronal growth cone filopodia by nitric oxide. J. Neurobiol. 39:168–185. Verma, A., D. J. Hirsch, C. E. Glatt, G. V. Ronnett, and S. H. Snyder. 1993. Carbon monoxide: A putative neural messenger. Science 259:381–384. Vincent, S. R. and H. Kimura. 1992. Histochemical mapping of nitric oxide synthase in the rat brain. Neuroscience 46:755–784. Weiler, E. and A. I. Farbman. 1997. Proliferation in
Downloaded from http://icb.oxfordjournals.org/ by guest on June 22, 2015
Mu¨ller, U. 1996. Inhibition of nitric oxide synthase impairs a distinct form of long-term memory in the honeybee, Apis mellifera. Neuron 16:541–549. Muller, U. and H. Hildebrandt. 1995. The nitric oxide/ cGMP system in the antennal lobe of Apis mellifera is implicated in integrative processing of chemosensory stimuli. Eur. J. Neurosci. 7:2240– 2248. Murphy, K. P. S. J. and T. V. P. Bliss. 1999. Photolytically released nitric oxide produces a delayed but persistent suppression of LTP area CA1 of the rat hippocampal slice. J. Physiol. London 515:453– 462. Murray, R. C. and A. L. Calof. 1999. Neuronal regeneration: Lessons from the olfactory system. Seminars in Cell & Develop. Biol. 10:421–431. Nakamura, H., K. Itoh, and M. Kawabuchi. 1999. NADPH-diaphorase and cytosolic urea cycle enzymes in the rat accessory olfactory bulb. J. Chem. Neuroanatomy 17:109–117. Nighorn, A., N. J. Gibson, D. M. Rivers, J. G. Hildebrand, and D. B. Morton. 1998. The nitric oxidecGMP pathway may mediate communication between sensory afferents and projection neurons in the antennal lobe of Manduca sexta. J. Neurosci. 18:7244–7255. Nikitin, E. S. and P. M. Balaban. 1999. Optical recording of odor-evoked responses in olfactory part of the brain of terrestrial mollusk Helix. Zhurnal Vysshei Nervnoi Deyatelnosti Imeni I P Pavlov 49:817–829. Nikitin, E. S. and P. M. Balaban. 2000. Optical recording of odor-evoked responses in the olfactory brain of the naive and aversively trained terrestrial snails. Learning & Memory 7:422–432. Okere, C. O., H. Kaba, and T. Higuchi. 1996. Formation of an olfactory recognition memory in mice: Reassessment of the role of nitric oxide. Neuroscience 71:349–354. Pape, H.-C. and R. Mager. 1992. Nitric oxide controls oscillatory activity in thalamocortical neurons. Neuron 9:441–448. Peschel, M., V. Straub, and T. Teyke. 1996. Consequences of food-attraction conditioning in Helix: A behavioral and electrophysiological study. J. Comp. Physiol. A 178:317–327. Philippides, A., P. Husbands, and M. O’Shea. 2000. Four-dimensional neuronal signaling by nitric oxide: A computational analysis. J. Neurosci. 20: 1199–1207. Pineda, J., J. H. Kogan, and G. K. Aghajanian. 1996. Nitric oxide and carbon monoxide activate locus coeruleus neurons through a cGMP-dependent protein kinase: Involvement of a nonselective cationic channel. J. Neurosci. 16:1389–1399. Ratte´, S. and R. Chase. 1997. Morphology of interneurons in the procerebrum of the snail Helix aspersa. J. Comp. Neurol. 384:359–372. Ratte´, S. and R. Chase. 2000. Synapse distribution of olfactory interneurons in the procerebrum of the snail Helix aspersa. J. Comp. Neurol. 417:366– 384. Renteria, R. C. and M. Constantine-Paton. 1999. Nitric oxide in the retinotectal system: A signal but not
ET AL.
GASEOUS OXIDES
OLFACTION
345
Zaitseva, O. V. 1991. Structural organisation of the tentacular sensory system in land pulmonates. In D. A. Sakharov and W. Winlow (eds.), Simpler nervous systems, pp. 238–257. Manchester University Press, New York. Zakharov, I. S., N. L. Hayes, V. N. Ierusalimsky, R. S. Nowakowski, and P. M. Balaban. 1998. Postembryonic neuronogenesis in the procerebrum of the terrestrial snail, Helix lucorum. J. Neurobiol. 35: 271–276. Zhuo, M., J. T. Laitinen, X. C. Li, and R. D. Hawkins. 1999. On the respective roles of nitric oxide and carbon monoxide in long-term potentiation in the hippocampus. Learning & Memory 6:63–76. Zufall, F. and T. Leinders-Zufall. 1997. Identification of a long-lasting form of odor adaptation that depends on the carbon monoxide/cGMP second messenger system. J. Neurosci. 17:2703–2712.
Downloaded from http://icb.oxfordjournals.org/ by guest on June 22, 2015
the rat olfactory epithelium: Age dependent changes. J. Neurosci. 17:3610–3622. Weiler, E., M. A. McCulloch, and A. I. Farbman. 1999. Proliferation in the vomeronasal organ of the rat during postnatal development. European J. Neurosci. 11:700–711. Wildemann, B. and G. Bicker. 1999. Developmental expression of nitric oxide/cyclic GMP synthesizing cells in the nervous system of Drosophila melanogaster. J. Neurobiol. 38:1–15. Wilson, R. I., A. Godecke, R. E. Brown, J. Schrader, and H. L. Haas. 1999. Mice deficient in endothelial nitric oxide synthase exhibit a selective deficit in hippocampal long-term potentiation. Neuroscience 90:1157–1165. Yang, Q. Z. and G. I. Hatton. 1999. Nitric oxide via cGMP-dependent mechanisms increases dye coupling and excitability of rat supraoptic nucleus neurons. J. Neurosci. 19:4270–4279.
AND